Rhizoma of Smilax china has been used as anti-inflammatory and analgesic, antiedemic agent in Korean folk medicine. In order to investigate the efficacy of anti-oxidati ve activity, the activity-guided fractionation and the isolation were performed. Each fractions (H2O fraction, 20%, 40%, 60%, 100% MeOH fractions and CHCl3 fraction) was examined anti-oxidant activity by 1,1- diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging potential. It was revealed that 40%, 20% MeOH fractions and H2O fractions have significant anti- oxidative activity. From 40% and 20% MeOH fractions two flavonoid glycosides and one procyanidin were isolated and elucidated apigenin-7-O-a-L-rhamnopyranosyl(1¡æ2)-¥â-D- glucopyranoside, apigenin-7-O-a-L-rhamnopyranosyl(1¡æ6)-¥â-D-glucopyranoside and catechin(4a¡æ6)epicatechin through their physicochemical data and IR, FAB-MS, 13C-NMR, and 1NMR analysis with authentics, respectively. The isolated compounds were examined by DPPH method. Apigenin-7-O-a-L-rhamnopyranosyl(1¡æ 2)-¥â-D-glucopyranoside and catechin (4a¡æ6) epicatechin showed powerful radical scavenging activities on DPPH radical among three compounds.
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